Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress.
- Author:
Ya-juan XU
1
;
Yi-ping ZHANG
;
Feng QIN
;
Chang-jing WU
;
You-rui LI
;
Qiang FU
Author Information
- Publication Type:Journal Article
- MeSH: Analysis of Variance; Animals; Cells, Cultured; Cytochalasin D; pharmacology; Cytoskeleton; physiology; Mice; Mice, Inbred BALB C; Osteoblasts; drug effects; metabolism; Proto-Oncogene Proteins c-fos; metabolism; RNA, Messenger; metabolism; Rheology; Stress, Mechanical
- From: Chinese Journal of Stomatology 2009;44(11):681-685
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.
METHODSBALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.
RESULTSIn control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).
CONCLUSIONSThe cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.
