Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress
10.3760/cma.j.issn.1002-0098.2009.11.011
- VernacularTitle:细胞骨架完整性对流体剪切力诱导成骨细胞c-fos基因mRNA和蛋白表达的影响
- Author:
Ya-Juan XU
1
;
Yi-Ping ZHANG
;
Feng QIN
;
Chang-Jing WU
;
You-Rui LI
;
Qiang FU
Author Information
1. 中山大学光华口腔医学院附属口腔医院
- Keywords:
Cytoskeleton;
Osteoblasts;
Genes,fos;
Fluid shear stress
- From:
Chinese Journal of Stomatology
2009;44(11):681-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.Methods BALB/c mouse primary osteoblasts were divided into four groups(according to fluid shear stress loaded or not and cytochalasin D used or not).The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA,c-fos protein and cytoskeleton,respectively.The data were analysed using two-way ANOVA.Results In control group and cytochalasin D group,fluid shear stress could significantly increase the expression levels of c-fos mRNA(0.1637±0.0303 and 0.0104±0.0070,respectively)and protein (177.14±9.37 and 150.95±6.17.respectively)in osteoblasts,compared with the unloaded osteoblasts of the control group and the cytochalasin D group(0.0057±0.0021 and 0.0032±0.0014,respectively for c-fos mRNA,and 117.96±4.11 and 119.77 ±5.19,respectively for protein,P＜0.05).Induced by the fluid shear stress.the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group,and the difference had statistical significance(P＜0.05).Conclusions The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.
