Integrity of multilayer of human periodontal ligament fibroblasts and human gingival fibroblasts grown on filter membrane of Transwell
10.3760/cma.j.issn.1002-0098.2009.11.013
- VernacularTitle:Transwell滤膜上人牙周膜及牙龈成纤维细胞细胞层完整性的研究
- Author:
Li-Qiang YU
1
;
Hong-Chen LIU
;
Ling-Ling E
;
Xia WU
;
Dong-Sheng WANG
Author Information
1. 解放军第309医院
- Keywords:
Fibroblasts;
Drug administration routes;
Transport
- From:
Chinese Journal of Stomatology
2009;44(11):690-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the integrity of multilayer of human periodontal ligament fibroblasts(HPDLF)and human gingival fibroblasts(HGF)on filter membrane of Transwell and to provide basis for the drug transcellular transport by the HPDLF and HGF in the hypothesis of delivering medicine to the periodontium and whole body through the root canal.Methods HPDLF and HGF derived from the primary culture were seeded on polycarbonate filter membrane of transwell respectively.After 1,2,3 and 4 weeks of culture.transepithelial electrical resistance(TEER)was detected and the growth of HPDLF and HGF observed by light microscope.After 2 weeks of culture,section of filter membrane where HPDLF and HGF lived was observed with light microscope and transmission electron microscope(TEM).and the permeability of the drug transport cell models was measurod with fluorescein sodium.Results HPDLF and HGF converged 1 week after inoculation.and the cells connected each other tighfly and completely 2 weeks later.Observation of section of filter membrane by light microscope and TEM revealed a stratified cell growth of HPDLF and HGF2 weeks after inoculation.and TEER of HPDLF and HGF were(56.14±7.43)and (57.34±7.62)Ω·cm~(-2) respectively. The values of TEER remained the same level until 4 weeks later.Two weeks after inoculation.the paracellular transport of fluorescein sodium was less than 1% after the cell models were incubated for 30 min.Conclusions Stratified cell layers of HPDLF and HGF grown on filter membrane of Transwell are analogous to periodontal membrane and gingiva 2 weeks after inoculation,the test results of permeability and TEER were consistent with the demands of development of cell models.HPDLF and HGF grown on filter membrane of Transwell could be used to study drug transcellular transport by HPDLF and HGF in vitro.
