Experimental study of human umbilical cord mesenchymal stem cells differentiating into periodontal ligament-like cells

VernacularTitle:人脐带间充质干细胞诱导为牙周韧带样细胞的实验研究
Author: Yun FU ¹ ; Xun HONG ; Jia LIU
Author Information

1. 中山大学光华口腔医学院附属口腔医院
Keywords: Coculture techniques; Mesenehymal stem cells; Periodontal ligament cells
From: Chinese Journal of Stomatology 2009;44(10):584-587
CountryChina
Language:Chinese
Abstract: Objective To investigate the possibility of human umbilical cord mesenehymai stem cells(hUCMSC)differentiating into human periodontal ligament cells(hPDLC)by establishing a cocuhure model in vitro.Methods Indifeet coculture model of hPDLC with hUCMSC Was established on Transwell system.The protein expression of bone sialoprotein(BSP),ostocalcin(OCN)and osteopontin(OPN)was examined by immunohistochemical staining.The changes of hUCMSC on molecular level were analyzed by Western blotting.Results hUCMSC was in the form of polygon and rhombus after induction by hPDLC.Immunohistochemistry staining and Western blotting showed that OCN and OPN were up-regulated[OCN:(0.88±0.21)vs(1.42.±0.17),OPN:(0.93±0.13)vs(1.43±0.22),P＜0.05]and BSP wag down regulated [(1.60.±0.09)vs(0.75±0.20),P＜0.05]after coculture.Conclusions hUCMSC call differentiate into hPDLC under certain conditions in vitro and hUCMSC may hopefully become the stem cells in periodontal tissue engineering.