Single cell gel electrophoresis of a magnesium alloy coated with beta-tricalcium phosphate.
- Author:
Yu-quan HAO
1
;
Li-li TAN
;
Ting-ting YAN
;
Xiu-lin YAN
;
Ke YANG
;
Hong-jun AI
Author Information
- Publication Type:Journal Article
- MeSH: Alloys; Calcium Phosphates; Coated Materials, Biocompatible; Electrophoresis, Agar Gel; Humans; Magnesium; Materials Testing
- From: Chinese Journal of Stomatology 2009;44(10):626-628
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the genotoxicity of a magnesium alloy coated with beta-tricalcium phosphate (beta-TCP).
METHODSFour groups were designed. In the first group, AZ31B magnesium alloy surface was coated with beta-TCP using chemical bath deposition, and in the second group magnesium alloy was tested. The other two groups were negative control (pure titanium) and positive control groups (0.5 mg/L bleomycin). Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups, and 60 cells from each group were analysed. Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample. Student-Newman-Keuls (SNK) test was used to compare results from 4 groups.
RESULTSThere were no significant differences in tail moment and tail DNA percentage between magnesium alloy group [(0.52 +/- 0.12), (6.82 +/- 1.81)%] and magnesium alloy coated with beta-TCP group [(0.51 +/- 0.12), (6.89 +/- 1.93)%, P > 0.05]. Tail moment and tail DNA percentage in negative group were (0.47 +/- 0.14) and (6.29 +/- 1.64)%, and tail moment and tail DNA percentage in positive group were (5.17 +/- 1.23) and (22.09 +/- 4.51)%.
CONCLUSIONSNo significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with beta-TCP.
