Effect of a Chinese herbal prescription on collagen I in rat's femur under simulated weightlessness.
- Author:
Su-min HU
1
;
Peng ZHOU
;
Qian FU
;
Jia-jia YANG
;
Xue-min GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen Type I; analysis; Drugs, Chinese Herbal; pharmacology; Femur; chemistry; drug effects; Immunohistochemistry; Male; Rats; Rats, Wistar; Weightlessness Simulation
- From: China Journal of Orthopaedics and Traumatology 2010;23(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of a Chinese herbal prescription on collagen I in rat's femur under simulated weightlessness.
METHODSThirty Wistar rats were randomly divided into 3 groups: blank control group (10 rats), tail suspension group (TS, 10 rats), TS with Chinese medicine group (10 rats). Rats in TS with Chinese medicine group took a Chinese herbal prescription (contains Radix Rehmanniae Praeparata, Radix Achyranthis Bidentatae, Radix Astragali, Radix Angelicae Sinensis, Concha Ostreae prepared by acetic acid)by oral administration. After 1 week adaption and 3 weeks tail suspending, rat's left femur was colleced, and collagen I in femur neck was detected by immunohistochemical method.
RESULTSCounts and integral optical density (IOD) of collagen I coloration decreased significantly in TS group (P < 0.001), but no significant change in TS with Chinese medicine group (P > 0.05), as compared with control group.
CONCLUSIONGeneration of collagen I become weaken under simulated weightlessness, while the Chinese herbal prescription is effective to prevent the change, thus biochemistry environment of bone calcium deposition may be improved by this Chinese herbal prescription under simulated weightlessness.
