Optimization of supercritical CO2 extraction of plantaginis semen oil by response surface methodology and studies on its antioxidant activity.

Wei XU; Weijian LING; Kedan CHU; Huang LI; Lidian CHEN; Yuqin ZHANG; Xianwen CHEN

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Optimization of supercritical CO2 extraction of plantaginis semen oil by response surface methodology and studies on its antioxidant activity.

Author: Wei XU ¹ ; Weijian LING ; Kedan CHU ; Huang LI ; Lidian CHEN ; Yuqin ZHANG ; Xianwen CHEN
Author Information

1. Pharmacy college, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China. xwfjtcm@sina.com
Publication Type:Journal Article
MeSH: Antioxidants; chemistry; isolation & purification; Carbon Dioxide; chemistry; Chromatography, Supercritical Fluid; methods; Cupressaceae; chemistry; Gas Chromatography-Mass Spectrometry; Plant Oils; chemistry; isolation & purification
From: China Journal of Chinese Materia Medica 2011;36(23):3253-3257
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThe optimum conditions of SFE-CO2 extraction of Plantaginis Semen oil (SPO), the composition of SPO and its antioxidant activities of SPO were all investigated in this paper.
METHODResponse surface method (RSM) was used to establish the mathematical model of SFE-CO2 extraction of SPO to obtain the optimum conditions based on Single factor experiments. Fatty acid compositions and contents of SPO were tested by GC-MS, and antioxidant activities of SPO were studied by DPPH and ABTS free radical elimination method.
RESULTThe optimum conditions obtained through RSM analysis were as follows: extraction tempreture 70 degrees C, extraction pressure 30 MPa, extraction time 120 min and flow rate 30 L x h(-1). Under the optimal condition, predicted value was 35.91%, while the experimental value was 35.07%. The experimental values agree with the predicted from the regression model with a relative error less than 5%. The main components of SPO were 9,12,15-octadecatrienoic acid, 8,11-octadecadienoic acid, octadecanoic acid and hexadecanoic acid. Most of the fatty acids were polyunsaturated fatty ones, whose quantities were obtained more than 88%. The IC50DPPH and IC50ABTS were 1.13, 3.57 g x L(-1) respectively in DPPH and ABTS assay.
CONCLUSIONOptimization of the extraction process by RSM of SPO is convenient and feasible. SPO has good antioxidant activity and is worth to develop for application.