Determination of o-methylflavinantine in a Tibetan medicine Meconopsis quintuplinervia by HPLC.
- Author:
Xia SHI
1
;
Fang CHENG
;
Mei GUO
;
Jing SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; chemistry; Morphinans; analysis; Papaveraceae; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(23):3290-3292
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of a Tibetan medicine Meconopsis quintuplinervia.
METHODA Hypersil-Keystone-C18 column (4.6 mm x 250 mm, 5 microm) was used with the isocratic elution of acetonitrile and 0.012% glacial acetic acid. The flow rate was 1.0 mL x min(-1), and the detection wavelength was set at 237 nm.
RESULTThe linear range of 0-methylflavinantine was 0.2-2.4 microg (r = 0.999 7). The average recovery was 96.26%.
CONCLUSIONThe developed method was reliable, and can be used for the quality control of M. quintuplinervia Regel.
