Study on HPLC fingerprint chromatograms of Arisaematis Rhizoma.
- Author:
Fen LUO
1
;
Dan LU
;
Yumei CHI
;
Hao WU
;
Hongli YU
Author Information
1. Nanjing University of Chinese Medicine, Nanjing 210046, China. luofen0227@163.com
- Publication Type:Journal Article
- MeSH:
Arisaema;
chemistry;
Chromatography;
Drugs, Chinese Herbal;
chemistry;
standards;
Quality Control;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2011;36(23):3302-3305
- CountryChina
- Language:Chinese
-
Abstract:
The fingerprint chromatograms of Arisaematis Rhizoma were established by HPLC. The analysis was performed on a Lichrospher C18 (4.6 mm x 200 mm, 5 microm) column with acetonitrile-water (containing 0.1% acetic acid) as mobile phase at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 270 nm, and the column temperature was 30 degrees C. The similarities of the fingerprint chromatograms were calculated over 0.9 between 11 batches of Arisaematis Rhizoma samples by analyzing 14 common peaks with adenosine as reference substance. However, their fingerprint chromatograms were significantly different from those of Pinellia pedatisecta and P. ternate. Adenine, hypoxanthine, xanthine, uridine, guanosine, adenosine, schaftoside, and isoschaftoside were identified by comparing the retention times and their ultraviolet spectra. The method is repeatable, exclusive and can be used for identification and evaluation of Arisaematis Rhizoma.
