Effect of down-regulation of GAT-1 on analgesic action of oxysophoridine.
- Author:
Jinxian GAO
1
;
Zhenhong YI
;
Guang YANG
;
Lin YAN
;
Lijun TAO
;
Shuansheng GAO
;
Qiuyan PAN
;
Yuanxu JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Analgesics; pharmacology; Animals; Brain; drug effects; metabolism; Down-Regulation; drug effects; Female; GABA Plasma Membrane Transport Proteins; genetics; metabolism; Gene Expression Regulation; drug effects; Male; Mice; RNA, Messenger; analysis; Spinal Cord; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(23):3315-3318
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of GABA transporter (GAT-1) on the analgesic action of oxysophoridine (OSR) in the central nervous system of mice.
METHODHot plate test was used to observe and analyze the effect of gamma aminobutyric acid and the inhibitor of GAT-1 (NO-711) on the analgesic action of oxysophoridine. Real time RT-PCR was used to investigate the influence of OSR on the expression of GAT-1 mRNA induced by formalin in spinal cord and brain of mice.
RESULTBoth GABA (2.0 mg x kg(-1), icv) and NO-711(0.125 mg x kg(-1), icv) enhanced the analgesic action of OSR (32.0 mg x kg(-1), iv) in the hot plate test, and the latencies was markedly increased (P < 0.05, P < 0.01). OSR (500.0 mg x kg(-1), iv) significantly inhibited the expression of GAT-1 mRNA induced by formalin (P < 0.05).
CONCLUSIONGAT-1 was involved in the analgesia effect of OSR and the down-regulation of GAT-1 mRNA enhanced the analgesic effect.
