Establishment of detection system of CNVs HMGR, SQS1, beta-AS synthase gene of Glycyrrhiza uralensis.
- Author:
Ying LIU
1
;
Dongji LIU
;
Chunsheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: DNA Copy Number Variations; DNA, Plant; Farnesyl-Diphosphate Farnesyltransferase; genetics; Glycyrrhiza uralensis; enzymology; genetics; Hydroxymethylglutaryl CoA Reductases; genetics; Molecular Typing; methods; Real-Time Polymerase Chain Reaction; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2012;37(3):283-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a stabilized and reliable detection system of CNVs of HMGR, SQS1, beta-AS gene of Glycyrrhiza uralensis.
METHODReal time PCR was used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis.
RESULTIn the quantitative detection experiments of HMGR, SQS1, beta-AS gene of G. uralensis, the change of value of C(t) was 25.82-25.88, 29.01-29. 08, 15.52-15.56, 19.06-19.08 respectively, the alue of SD was 0.033, 0.032, 0.024, 0.011 respectively, and the value of CV was 0.12%, 0.22%, 0.16%, 0.06% respectively.
CONCLUSIONThe repeatability of detection system of Real time PCR was stabilized and reliable, and the method could be used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis.
