Study on once sampling quantitation based on information entropy of ISSR amplified bands of Houttuynia cordata.
- Author:
Haiqin WANG
1
;
Wenlong LIU
;
Fuyuan HE
;
Zuohong CHEN
;
Xili ZHANG
;
Xianggui XIE
;
Jiaoli ZENG
;
Xiaopeng DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; DNA, Plant; Genetic Variation; Houttuynia; genetics; Microsatellite Repeats; genetics
- From: China Journal of Chinese Materia Medica 2012;37(3):288-292
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the once sampling quantitation of Houttuynia cordata through its DNA polymorphic bands that carried information entropy, from other form that the expression of traditional Chinese medicine polymorphism, genetic polymorphism, of traditional Chinese medicine.
METHODThe technique of inter simple sequence repeat (ISSR) was applied to analyze genetic polymorphism of H. cordata samples from the same GAP producing area, the DNA genetic bands were transformed its into the information entropy, and the minimum once sampling quantitation with the mathematical mode was measured.
RESULTOne hundred and thirty-four DNA bands were obtained by using 9 screened ISSR primers to amplify from 46 strains DNA samples of H. cordata from the same GAP, the information entropy was H=0.365 6-0.978 6, and RSD was 14.75%. The once sampling quantitation was W=11.22 kg (863 strains).
CONCLUSIONThe "once minimum sampling quantitation" were calculated from the angle of the genetic polymorphism of H. cordata, and a great differences between this volume and the amount from the angle of fingerprint were found.
