Inhibitory effect and mechanism of procyanidin from vaccinium on isoprenaline-induced myocardial fibrosis in rats.
- Author:
Hongyan FAN
1
;
Yanchun WANG
;
Kuang REN
;
Raosheng GU
;
Nan SHEN
;
Ying AN
;
Yue ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensins; blood; Animals; Antioxidants; administration & dosage; pharmacology; Biflavonoids; administration & dosage; pharmacology; Catechin; administration & dosage; pharmacology; Endomyocardial Fibrosis; chemically induced; drug therapy; metabolism; Female; Isoproterenol; adverse effects; Male; Malondialdehyde; metabolism; Nitric Oxide; blood; Proanthocyanidins; administration & dosage; pharmacology; Rats; Rats, Wistar; Vaccinium; chemistry; Ventricular Remodeling; drug effects
- From: China Journal of Chinese Materia Medica 2012;37(3):344-347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the effect and mechanism of procyanidin foom vaccinium (PC) on myocardial fibrosis in rats.
METHODThe myocardial fibrosis model in rats was built by subcutaneous injection of 5 mg x kg(-1) x d(-1) of isoprenaline (Iso) for 7 days in vivo while intragastrically administrating PC 100, 200 and 400 mg x kg(-1) x d(-1) for 14 days. Following the model was established, myocardial indexes (heart weight/body weight, HW/BW and left ventricalar weight/body weight, LVW/BW) were measured. Besides, angiotensin II and I , III collagen quantification levels in blood serum were determined respectively by ELISA. The change in the content of nitric oxide (NO) in blood serum was determined with colorimetry. The content of malondialdehyde (MDA) in left ventricle was assayed with spectrophotometry. The contents of lactate dehydrogenase (LDH) and creatine kinase (CK) in blood serum were detected with automatic biochemistry analyzer.
RESULTCompared with the control group, the myocardial indexes, the contents of I , III collagen quantification, angiotensin II in blood serum and malondialdehyde in left ventricle were markedly increased and the content of nitric oxide in blood serum was decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly increased in Iso model group (P<0.05 or P<0.01). Compared with the model group, the myocardial indexes were decreased, the contents of I , III collagen quantification, angiotensin II in blood serum were reduced in PC 200 and 400 mg x kg(-1) x d(-1) groups (P<0.05 or P<0.01). The content of nitric oxide in blood serum was increased, the content of malondialdehyde in left ventricle was markedly decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly decreased in PC three groups (P<0.05 or P<0.01).
CONCLUSIONPC could inhibit collagen synthesis by decreasing angiotensin II level and increasing the content of nitric oxide and antioxidation, and thereby inhibiting myocardial fibrosis and protect myocardium in rats.
