Effect of jinmaitong capsule on inducible nitric oxide synthase and nitrotyrosine in diabetic rats.
- Author:
Qing SUN
1
;
Xiaochun LIANG
;
Puyan WANG
;
Bowu LI
;
Li ZHAO
;
Wenzhi HUANG
;
Yandong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; Body Weight; drug effects; Capsules; Diabetes Mellitus, Experimental; drug therapy; metabolism; pathology; Diabetic Neuropathies; drug therapy; metabolism; pathology; Drugs, Chinese Herbal; administration & dosage; pharmacology; Enzyme Activation; drug effects; Male; Nitric Oxide Synthase Type II; metabolism; Rats; Rats, Sprague-Dawley; Sciatic Nerve; drug effects; metabolism; pathology; Tyrosine; analogs & derivatives; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(3):348-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Jinmaitong capsule (JMT), a compound traditional Chinese medicine, on expressions of inducible nitric oxide synthase (iNOS) and nitro tyrosine (NT) protein in streptozocin-induced diabetic (STZ-DM) rats.
METHODIntraperitoneal injection of streptozocin in rats to establish a model. STZ-DM rats were randomly divided into the model control group (distilled water), the small-dose JMT group (JMT at dose of 0.45 g x kg(-1) x d(-1)), the medium-dose JMT group (JMT at dose of 0.88 g x kg(-1) x d(-1)), the large-dose JMT group (JMT at dose of 1.75 g x kg(-1) x d(-1)) and Vitamin C group (VC at dose of 0.05 g x kg(-1) x d(-1)). Ten normal rats with matching weight and age were selected as the normal control group (distilled water). After intragastric administration for 16 weeks, the expressions of iNOS and NT in sciatic nerve were detected by the immunohistochemistry method.
RESULTThe expression levels of iNOS and NT protein in diabetic rats were higher than those in normal rats (P<0.05, P<0.01). Compared with the model group, the levels of iNOS and NT protein in JMT and VC groups were significantly decreased (P<0.05, P<0.01). Particularly, the medium-dose JMT group showed a better effect than the VC group (P<0.05, P<0.01).
CONCLUSIONJMT could down-regulate the expressions of iNOS and NT protein of sciatic nerve in diabetic rats.
