Protective effect of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats.
- Author:
Feng GAO
1
;
Guibo SUN
;
Xiaoyu REN
;
Yuanyuan NIE
;
Jing SUN
;
Meng QIN
;
Xiaobo SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzofurans; administration & dosage; pharmacology; Drugs, Chinese Herbal; administration & dosage; pharmacology; Hemodynamics; drug effects; In Vitro Techniques; L-Lactate Dehydrogenase; metabolism; Male; Malondialdehyde; metabolism; Myocardial Reperfusion Injury; metabolism; prevention & control; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; metabolism; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(3):358-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect and mechanism of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats.
METHODForty-eight SD rats were divided into 6 groups randomly(n = 8): the control group, the positive administration group (verapamil 150 microg x L(-1)), and high, middle and low-dose salvianolic acid B groups (10, 5, 2.5 mg x L(-1)). The myocardial ischemia/reperfusion injury model was established using the Langendorff method, re-perusing isolated working hearts for 30 min after ischemia for 25 min. A water-bag catheter was inserted in rat left atrium for recording the effect of salvianolic acid B on hemodynamics indexes-AST, LDH, SOD and MDA.
RESULTVarious group with different doses showed that salvianolic acid B decreased AST, release of LDH and formation of MDA and increased SOD activity.
CONCLUSIONSalvianolic acid B showed a protective effect on myocardial ischemia/reperfusion injury. Its mechanism may be related with improvement of cardiac contractility, cleaning of oxygen free radicals and reduction of lipid peroxidation.
