Study on andrographolide-induced apoptosis of Candida albicans biofilm dispersion cells.
- Author:
Changzhong WANG
1
;
Ning HAN
;
Zhenhua XU
;
Huijuan CHENG
;
Yan GUAN
;
Yun YUN
;
Yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antifungal Agents; pharmacology; Apoptosis; drug effects; Biofilms; Candida albicans; drug effects; physiology; Diterpenes; pharmacology; Membrane Potential, Mitochondrial; drug effects; Reactive Oxygen Species; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(3):362-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the effect of andrographolide on apoptosis of Candida albicans biofilm dispersion cells.
METHODThe morphological changes of apoptotic C. albicans biofilm cells were observed by using Hoechst 33258 staining Fluorescence microscope; changes of mitochondrial membrane potential (MMP) of C. albicans biofilm cells were detected by rhodamine 123 staining flow cytometry; and reactive oxygen species (ROS) was detected by DHR staining flow cytometry.
RESULT1 000, 100 micromol x L(-1) of andrographolide could cause pyknosis and dense staining of C. albicans biofilm cells, 1 000, 100, 10 micromol x L(-1) of andrographolide could decrease MMP and increase ROS of C. albicans biofilm cells.
CONCLUSIONAndrographolide of appropriate concentrations could induce apoptosis of dispersion cells of C. albicans biofilms.
