Study on absorption kinetics of betulic acid in rat's intestines.
- Author:
Huijun XU
1
;
Xiaohua REN
;
Yingfeng DU
;
Lantong ZHANG
;
Tao LI
;
Ying GE
;
Huijie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-HIV Agents; pharmacokinetics; Antineoplastic Agents, Phytogenic; pharmacokinetics; Chromatography, High Pressure Liquid; Intestinal Absorption; Intestines; metabolism; Kinetics; Male; Rats; Rats, Sprague-Dawley; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Triterpenes; pharmacokinetics
- From: China Journal of Chinese Materia Medica 2012;37(3):377-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC-DAD method for determining concentrations of betulic acid and phenol red in intestinal circulation liquid, and probe into the absorption kinetic characteristics of betulic acid at different intestine segments in rats and the effect of different drug concentrations on absorption.
METHODThe rat intestinal absorption model was established to detect the impact of absorption site, drug concentration and pH value on drug absorption.
RESULTWithin the range from 75-125 mg x L(-1), the absorption rate and the quality concentration of betulic acid had a linear relation, with Ka value keeping unchanged. The absorption rate for each intestinal segment showed no remarkable difference, with Ka values in duodenum, jejunum, ileum and colon being (0.151 +/- 0.0049), (0.159 +/- 0.0056), (0.156 +/- 6.0083), (0.149 +/- 0.0041) h(-1), respectively.
CONCLUSIONBetulic acid is proved to be well absorbed in intestines marked by no specific absorption site in the intestine. The absorption mechanism of the drug conforms to passive transport mechanism and first-order kinetics. The bioavailability of betulic acid preparation can be increased by enhancing the dissolution rate and the solubility.
