Phamacokinetic study of bergapten in rats plasma by LC-MS/MS.
- Author:
Yufeng MA
1
;
Wen LI
;
Fangdi HU
;
Yikai SHI
;
Yingyan BI
;
Shilan FENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, Liquid; Male; Methoxsalen; analogs & derivatives; blood; pharmacokinetics; Rats; Rats, Sprague-Dawley; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(3):381-383
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine bergapten's concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.
METHODBlood samples were separated on a Hypersil ODS column (4.6 mm x 250 mm, 5 mm) at a temperature of 30 degrees C, and mobile phase consisted of water and methanol (22.5: 77.5) at a flow rate of 0.8 mL x min(-1).
RESULTThe methodological study showed a good linear relationship of 8.12-162.4 g x L(-1) (r = 0.9999). The inner and inter-days relative standard deviations were both less than 10% , indicating legitimate precise and accuracy to the requirement of biological sample analysis.
CONCLUSIONThe method is suitable for in vivo quantitative analysis for bergapten due to its accuracy, sensitivity and specificity. The pharmacokinetic process in rats forms a two-compartment model with first-order absorption.
