Study on plant regeneration from somatic embryos of vulnerable medicinal plant Glehnia littoralis.
- Author:
Hongbo LI
1
;
Dan SUN
;
Yongchang HUANG
;
Yinbo MA
;
Zhongyun PIAO
Author Information
- Publication Type:Journal Article
- MeSH: Apiaceae; physiology; Endangered Species; Plant Somatic Embryogenesis Techniques; Plants, Medicinal; physiology; Regeneration; Seeds; physiology
- From: China Journal of Chinese Materia Medica 2012;37(4):434-437
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cause of the seeds dormancy of Glehnia littoralis in vitro and to establish plant regeneration methods via somatic embryos.
METHODThe effects of endosperm and exogenous hormone on the seed dormancy breaking of G. littoralis and the effect of hormone concentration on embryonic callus induction and plant regeneration via somatic embryos were observed,
RESULTSThe germination rate of the seeds with 1/3 endosperm was the highest which achieved 31%. TDZ, 6-BA and GA3 treatment could not break seed dormancy but easily lead to abnormal seedlings. Embryogenic callus induction rates was up to 57% on MS supplemented with 1.0 mg x L(-1) 2,4-D. After 20 days culture, embryogenic calli were transferred to MS medium and cotyledonary embryos were formed in 40 days. The regenerated plants were obtained in 20 days.
CONCLUSIONAn effective system of plant regeneration of G. littoralis was established in this study.
