Study on extraction and immune regulation of polysaccharides from Marsdenia tenacissima.
- Author:
Xiufeng ZHU
1
;
Yuanyuan LI
;
Yonghua YUE
;
Xianrong LI
;
Xuliang HAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Body Weight; drug effects; CD4-CD8 Ratio; Chemical Fractionation; methods; Interleukin-2; blood; Interleukin-6; blood; Marsdenia; chemistry; Mice; Polysaccharides; immunology; isolation & purification; pharmacology; Spleen; immunology; Temperature; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(4):457-460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the extraction technology and determine the immune activity of polysaccharides from Marsdenia tenacissima.
METHODThe optimum extraction technology of polysaccharide from M. tenacissima was detected by orthogonal experiments with the extraction rate of polysacchride and the total sugar content as indicators. The immunocompromised mice model was established by intraperitoneal injection cyclophosphamide to detected the content of IL-2, IL-6 in serum, CD4+, CD8+ in the peripheral blood by ELISA and flow cytometry, respectively.
RESULTBy the extraction rate of polysaccharide, the sequence of seriousness of all affecting factors from high to low was extracting times, temperature, heating time and water ratio. By the total sugar content, the sequence was temperature, extracting times, water ratio and heating time. Compared with the model group, the pleen index, IL-2, IL-6, CD4/CD8+ were increased significantly (P < 0.05) in the 0.14 g x kg(-1) group and 0. 28 g x kg(-1) group.
CONCLUSIONT he optimum extraction condition was as follows: extraction three times/1.5 hours at 100 degrees C with 1:8 ratio of M. tenacissima to water. The polysaccharide of M. tenacissima can enhance the cellular immune and humoral immune.
