Effect and mechanism of quercetin on proliferation and apoptosis of human osteosarcoma cell U-2OS/MTX300.
- Author:
Junqiang YIN
1
;
Xianbiao XIE
;
Qiang JIA
;
Jin WANG
;
Gang HUANG
;
Changye ZOU
;
Jingnan SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Bone Neoplasms; drug therapy; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Humans; Osteosarcoma; drug therapy; pathology; Proto-Oncogene Proteins c-akt; metabolism; Quercetin; pharmacology
- From: China Journal of Chinese Materia Medica 2012;37(5):611-614
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect and mechanisms of quercetin (Qu) on proliferation and apoptosis of human methotrexate resistant osteosarcoma cell U-2OS/MTX300.
METHODMTT assay was used to observe cell proliferation. The apoptosis was examined by using Annexin V/PI staining. Western blot of mitochondrial membrane potential and cytochrome c were used to detect mitochondria spoptosis pathway. The protein expressions related to Akt pathway was detected by continuous activated Akt transient transfection and western blot.
RESULTQu can obviously inhibit the growth of human MTX resistant osteosarcoma cell U-2OS/MTX300 cells in a dose- and time-dependent manner. Annexin V/PI staining showed obvious cell apoptosis. Reduction of mitochondrial membrane potential, release of mitochondrial cytochrome c to cytosol and dephoshorylation of Akt were observed after Qu treatment.
CONCLUSIONQu can inhibit proliferation and induce apoptosis of human MTX resistant osteosarcoma cell U-2OS/MTX300, which may be related with mitochondrial apoptosis pathway and Akt activity.
