Role of p38 mitogen activated protein kinase on cyclic stretch in human facial hypertrophic scar fibroblasts differentiation into myofibroblasts
10.3760/cma.j.issn.1002-0098.2013.10.009
- VernacularTitle:p38丝裂原活化蛋白激酶在周期性牵张力诱导人增生性瘢痕成纤维细胞向肌成纤维细胞分化中的作用
- Author:
Qi-Cui DU
1
;
Wen-Lin XIAO
;
Ling-Fa XUE
;
Shuang-Yi WANG
;
Jin YUE
;
Yao-Xiang XU
Author Information
1. 聊城市人民医院口腔修复科
- Keywords:
p38 Mitogen-activated protein kinases;
Stress,mechanical;
Fibroblasts;
Cell transdifferentiation
- From:
Chinese Journal of Stomatology
2013;48(10):615-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the signal transduction mechanism of p38 mitogen activated protein kinase (p38MAPK) in human facial hypertrophic scar fibroblast (FB) differentiation into myofibroblasts(MFB).Methods Fibroblasts of primary culture were simple randomly assigned into two groups:cyclic stretch (control group) and cyclic stretch pre-treated with SB203580 (experimental group).Expression of P-p38MAPK and α-smooth muscle actin (α-SMA) protein were examined using Western blotting and expression of transforming growth factor β1 (TGF-β1) mRNA and α-SMA mRNA were examined using reverse transcription PCR(RT-PCR).Results In control group,the expressions of α-SMA,p38MAPK,TGF-β1 mRNA and α-SMA mRNA(0 h:0.134 ± 0.011,0.239 ± 0.015,0.214 ± 0.018,0.252 ± 0.010 ; 6 h:0.152±0.014,0.287 ±0.016,0.288 ±0.011,0.277 ±0.013; 12 h:0.172 ±0.017,0.320 ±0.017,0.335 ±0.013,0.297 ± 0.006),were significantly increased with loading time (6 h ＞ 0 h; 12 h ＞ 0 and 6 h).In experimental group (pre-treated with SB203580),the expressions of α-SMA,p38MAPK,TGF-β1 mRNA,α-SMA mRNA(6 h:0.116 ±0.017,0.128 ±0.016,0.134 ±0.014,0.163 ±0.009; 12 h:0.149 ± 0.013,0.136 ±0.018,0.144 ±0.013,0.187 ±0.010) on corresponding time decreased sharply compared with those in control groups (6,12 h).Conclusions The human facial hypertrophic scar fibroblasts differentiation in response to cyclic stretch was mediated by p38MAPK phosporylation.
