Expression of transient receptor potential vanilloid 3 ion channel protein in human odontoblasts
10.3760/cma.j.issn.1002-0098.2013.11.009
- VernacularTitle:瞬时感受器电位香草酸受体3离子通道蛋白在人成牙本质细胞中的表达
- Author:
Chun-Yun LIANG
1
;
Sheng WU
;
De-Yu HU
;
Ke-Hua QUE
Author Information
1. 300070,天津医科大学口腔医院牙体牙髓科
- Keywords:
TRPV3 cation channels;
Immunohistochemistry;
Odontoblasts
- From:
Chinese Journal of Stomatology
2013;48(11):679-682
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of transient receptor potential vanilloid 3 (TRPV3) ion channel protein in human odontoblasts (OD).Methods Twenty intact and healthy third molars extracted for orthodontic purpose were included.The quality of dental tissue sections was determined through HE staining,and the OD layer was further determined by dentin sialophosphoproteins (DSPP) antibody staining,and finally the expression of TRPV3 ion channel protein in human dental pulp tissue was examined by TRPV3 ion channel protein-specific antibody.The expression of TRPV3 channel proteins in human OD at different part of dental pulp was compared using Image Pro Plus(IPP) and SPSS software.Results TRPV3 channel protein expressed on the cell body of OD in the coronal and root pulp,and the expression in the coronal pulp was significantly higher than that in the root pulp.The TRPV3 protein also expressed at the odontoblastic process,with the higher expression in the crown (IA =2516 ± 162)than in the root (IA =2224 ± 150) and external root (IA =2121 ± 92) (P ＜ 0.05),but the expression between the lateral root area and external root area was not significantly different (P ＞ 0.05).Conclusions Human odonoblasts expressed TRPV3 ion channel protein and the expression level was different at different part of dental pulp OD.
