Expressions of protease-activated receptors in human gingival fibroblasts and its functions in periodontitis
10.3760/cma.j.issn.1002-0098.2014.01.006
- VernacularTitle:牙龈成纤维细胞表达蛋白酶激活受体的类型及作用研究
- Author:
Diya ZHANG
1
;
Lingjing HU
;
Shenglai LI
;
Lieping SHENG
;
Zheng CAO
;
Lili CHEN
Author Information
1. 浙江大学医学院附属邵逸夫医院牙科
- Keywords:
Fibroblasts;
Receptors,proteinase-activated;
Interleukin-6;
Gingipain R;
Periodontitis
- From:
Chinese Journal of Stomatology
2014;49(1):21-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression types of protease-activated receptors (PAR) in human gingival fibroblasts(HGF) and the functions of PAR in periodontitis.Methods Primary HGF were cultured.Reverse transcription PCR(RT-PCR) was used to detect the expression of PAR in HGF.Recombinant gingipain R (rRgp) was applied to HGF.The change of PAR expression on the cell surface was analyzed by real-time quantitative RT-PCR,and enzyme-linked immunosorbent assay (ELISA) was used to detect the change of the interleukin(IL)-6 production from HGF.The results of RT-PCR and ELISA were statistically analyzed using the two independent samples t-test of SPSS10.0 software.Results HGF expressed PAR-1 and PAR-3.The expression of PAR-1 and PAR-3 changed after two rRgp treatment with HGF cells.The relative expression of PAR-1 was decreased from 1.04 ± 0.31 to 0.67 ± 0.11 and 0.31 ±0.11.The relative expression of PAR-3 was decreased from 1.01 ±0.44 to 0.79 ±0.13 and 0.44 ± 0.12 (P ＜ 0.05).The level of IL-6 was increased after rRgp treatment for 8 h.The control group was (18.77 ±4.09) μg/L,the rRgp treatment groups were (179.36 ± 15.81) and (320.56 ± 26.19) μg/L respectively.Conclusions HGF expressed PAR-1 and PAR-3 and were involved in periodontal inflammation.
