Porphyromonas gingivalis infection enhanced the adhesion of monocytes to endothelial cells
10.3760/cma.j.issn.1002-0098.2014.03.006
- VernacularTitle:牙龈卟啉单胞菌感染对单核细胞与人脐静脉内皮细胞黏附的影响
- Author:
Caixia ZHANG
1
;
Jie SONG
;
Jingjing LI
;
Xin GAO
;
Weibin SUN
;
Kuanyu LI
Author Information
1. 210093,南京大学医学院细胞生物学和遗传学教研室·江苏省医学分子技术重点实验室
- Keywords:
Porphyromonas gingivalis;
Monocytes;
Endothelial cells;
Cell adhesion molecules
- From:
Chinese Journal of Stomatology
2014;49(3):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe if Porphyromonas gingivalis (Pg) infection could enhance the adhesion of human monocytic cell line (THP-1) to human umbihcal vein endothelial cells (HUVEC).Methods PgATCC33277 was cultured in anaerobic jar,and THP-1 was infected with various concentrations of PgATCC33277 at multiplicity of infection(MOI) of 1:100 for 8 and 24 hours,respectively.Mter removal of the free Pg,THP-1 cells were cocultured with HUVEC for 1 hour to observe the adhesion of THP-1 to HUVEC.HUVEC with adhesive THP-1 cells were co-cultured for additional 23 hours.The medium and cells were separately collected.The expression of related chemotactic cytokine [monocyte chemotactic protein 1 (MCP-1) and interleukin 8 (IL-8)] and intercellular adhesion molecule-1 (ICAM-1) were detected with enzyme-linked immunosorbent assay.Results The adhesion of THP-1 to HUVEC was enhanced (13.8％-35.2％,P=0.006) and the expression of ICAM-1 of HUVEC was increased from (132.5 ±7.7) to (164.9 ±9.1) ng/L (P=0.005) after infection for 24 hours by Pg.Both of the secreted MCP-I and IL-8 elevated after infection of Pg for 24 hours from (183.2 ± 3.1) to (221.0 ± 4.9) ng/L (P =0.012) and from (587.2 ±5.1) to (787.2 ± 10.3) ng/L (P =0.002),respectively.Conclusions Pg could enhance the adhesion of monocytes to endothelial cells and stimulate the inflammation,suggesting that Pg infection may be one of the risk factors in promoting the development of atherosclerosis.
