Biological effect of inhibition of Notch signaling pathway on human dental pulp cells
10.3760/cma.j.issn.1002-0098.2014.04.004
- VernacularTitle:抑制Notch信号通路对人牙髓细胞的生物学效应
- Author:
Xiaoying ZOU
1
;
Heng ZHUANG
;
Lin YUE
;
Xuejun GAO
Author Information
1. 100081,北京大学口腔医学院·口腔医院牙体牙髓科
- Keywords:
Receptors,Notch;
Cell biology;
Cell aging;
Dental pulp cells
- From:
Chinese Journal of Stomatology
2014;49(4):210-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Notch signaling pathway on human dental pulp cells.Methods The γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester(DAPT) was applied to inhibit the Notch signaling pathway of human dental pulp cells.The solvent dimethly sulfoxide (DMSO) served as the negative control.Senescence conditions were evaluated by cells morphology changes,the alkaline phosphatase (ALP) expression and its activity,senescenceassociated β-galactosidase(SA-β-Gal) expression and the senescence related gene p53 expression.Results After inhibition of the Notch signaling pathway,morphology changes,including flatter cells,larger plasma area,were seen in the 10th passage human dental pulp cells.ALP expression and activity showed a significant decrease at the 8th passage after inhibition (35.36 ± 2.55) U/g,compared with the negative control group[(49.76 ±4.30) U/g] (t =4.989,P =0.008).SA-β-Gal-positive cells could be seen as early as the 8th passage and more positive cells were evident at the 10th passage.The relative expression level of p53 gene was elevated in the 10th passage cells(1.7 ±0.4) compared with the negative control group(1.0) (t =3.581,P =0.012).Conclusions Human dental pulp cells became senescent at earlier passages after inhibition of Notch signaling pathway.Notch signaling pathway may affect life cycle of human dental pulp cells.
