Effect of Bortezomib on Proliferation, Apoptosis and SHP-2 Gene Expression of Lymphoma Cells.
- Author:
Li-Cai JIANG
1
;
Jian-Min LUO
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Bortezomib; Cell Line, Tumor; Cell Proliferation; Flow Cytometry; Gene Expression Regulation, Leukemic; Humans; Lymphoma; genetics; pathology; Protein Tyrosine Phosphatase, Non-Receptor Type 11
- From: Journal of Experimental Hematology 2015;23(4):1026-1029
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Bortezomib on proliferation, apoptosis and SHP-2 gene expression of lymphoma Jurkat cells and Raji cells.
METHODSMethylthiazoly tetrazolium assay (MTT) was used to observe the proliferation of Jurkat cells and Raji cells treated with bortezomib in different doses. Cell apoptosis was detected by morphological examination and flow cytometry. The level of SHP-2 mRNA expression before and after the treatment with bortezomib was measured by RT-PCR.
RESULTSBortezomib could inhibit the proliferation of Jurkat and Raji cells and induce their apoptosis with time-and dose-dependent manner. After treatment with 5-100 nmol/L bortezomib, the expression of SHP-2 in Jurkat cells and Raji cells was upregulated.
CONCLUSIONBortezomib can inhibit the proliferation and induc the apoptosis of Jurkat and Raji cells obviously, upregulate the expression of SHP-2 mRNA, suggesting that the SHP-2 may participate in regulation of bortezomib induced apoptosis of Jurkat cells and Raji cells.
