Research Progress on Cytometric Bead Assay for Platelet Antibody Detection.
10.7534/j.issn.1009-2137.2015.04.059
- Author:
Yun LING
1
;
Xin KONG
2
;
Bao-An CHEN
3
Author Information
1. Department of Hematology (Key Discipline of Jiansu Province), Zhongda Hospital Affiliated to Southeast University Medical college, Nanjing 210009, Jiangsu Province, China.
2. Department of Hematology, The Third Affiliated Hospital of Soochow University, Changzhou First People's Hospital, Changzhou 213000, Jiangsu Province, China.
3. Department of Hematology (Key Discipline of Jiansu Province), Zhongda Hospital Affiliated to Southeast University Medical college, Nanjing 210009, Jiangsu Province, China. E-mail: cba8888@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Antibodies;
Antibodies, Monoclonal;
Antigens, Human Platelet;
Blood Platelets;
Humans;
Megakaryocytes;
Thrombocytopenia
- From:
Journal of Experimental Hematology
2015;23(4):1208-1211
- CountryChina
- Language:Chinese
-
Abstract:
Anti-platelet specific antibody is one of the most important reasons leading to thrombocytopenia and megakaryocyte dysmaturity. The detection of platelet autoantibodies is an important step in the diagnosis of ITP because of the absence of specific clinic feature. The monoclonal antibody-specific immobilization of platelet antigens (MAIPA) has become a "gold standard" for determination of PLT specific antibody, which has high specificity and low sensitivity. However, this assay is time-consuming and tedious work. Routine use of this assay in hospital is difficult. Recently, some researches reporded the cytometric bead assay that has higher sensitivity than MAIPA, and so probably solves the problem of time-consuming partly, that also can use different beads for simultaneous detection. This review focuses on recent progress of the cytometric bead assay.
