Role of CyclinD1/IgH Detection by FISH in Differential Diagnostic Significance between Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia.
- Author:
Guo-Ping LI
1
;
Wan-Zi CHEN
2
;
Hui-Fang HUANG
3
;
Jia-Di CHEN
2
;
Xiao-Lan LIN
2
;
Qiang FU
2
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow; pathology; Diagnosis, Differential; Humans; Immunophenotyping; Leukemia, Lymphocytic, Chronic, B-Cell; diagnosis; metabolism; Lymphoma, Mantle-Cell; diagnosis; metabolism; Oncogene Proteins, Fusion; metabolism
- From: Journal of Experimental Hematology 2015;23(5):1314-1317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of CyclinD1/IgH detection by FISH in diferential diagnosis between mantle cell lymphoma (MCL) and chronic lymphocytic leukeamia (CLL).
METHODSThe FISH detection was performed for CyclinD1/IgH fusion gene. A comprehensive analysis was carried out for clinical features, such as age, sex , WBC count and lymphocyte count, the bone marrow morphology and immunohistochemical staining were carried for CyclinD1/IgH.
RESULTSIt is often difficult to distinguish MCL from CLL by bone marrow morphology, when the cell morphology was not typical; there was no difference in age, sex, WBC count and lymphocyte count between MCL and CLL groups; 9 out of 52 patients were diagnosed as MCL, and the direction of CyclinD1/IgH by FISH was positive in 7 of 9 MCL, while 3 of the 7 patients were negative by immunohistochemical staining for CyclinD1.
CONCLUSIONDetection of CyclinD1/IgH by FISH can be used as a specific and feasible method for differential diagnosis of mantle cell lymphoma from chronic lymphocytic leukeamia.
