Identification of A Novel HLA Allele DRB1 * 16:36 by Sequencing-Based Typing.

Li-jun LU; Wei-ze Zuo; Wan-jiang Zhang; Le Zhang; Fang WU; Jiang-dong WU

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Identification of A Novel HLA Allele DRB1 * 16:36 by Sequencing-Based Typing.

Author: Li-Jun LU ^{1
,

2} ; Wei-Ze ZUO ³ ; Wan-Jiang ZHANG ¹ ; Le ZHANG ¹ ; Fang WU ¹ ; Jiang-Dong WU ¹ ;
Author Information

1. State Key Laboratory, Ministry of Education, Shihezi University Medical College
2. Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
3. Department of Infectiou Disease, The First Affiliated Hospital of Shihezi University Medical College, Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
Publication Type:Journal Article
MeSH: Alleles; Base Sequence; DNA Primers; Exons; Female; Genotype; HLA-DRB1 Chains; genetics; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA
From: Journal of Experimental Hematology 2015;23(5):1455-1458
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo identify a novel HLA allele DRB1 * 16:36 from a Uygur woman.
METHODSPCR-SBT technology was applied to the extracted DNA for genotyping, and a possible new gene was sequenced by using sequence specific primers and single stranded SBT. This novel allele was compared with known most homologous gene sequences and their difference was analyzed.
RESULTSThis novel allele was different from HLA alle DRB1 * 16:23, and had highest similarity in 2 nucleotides at position 227 A→T and 236 T→C in exon 2, resulting in 3 amino acid changes from Tyr to Phe at codon 47 and Val to Ala at codon 50. The sequence of this novel allele had been submitted to GenBank.
CONCLUSIONThis HLA allele DRB1 * 16:23 has been confirmed to be a novel allele, and has been officially named DRB1 * 16:36 by the World Health Organization (WHO) Nomenclature Committee in May 2015.