Arginase Level in Suspended Red Blood Cells Storaged for Different Time.
- Author:
Li-Ping FAN
1
;
Hao-Bo HUANG
1
;
Shi-Jin WEI
1
;
Dan-Hui FU
1
;
Feng ZENG
1
;
Qing-Hua HUANG
1
;
Jin-Quan HONG
2
Author Information
- Publication Type:Journal Article
- MeSH: Arginase; chemistry; Blood Preservation; Erythrocytes; enzymology; Humans; Peroxidase; chemistry; Plasma; enzymology; Time Factors
- From: Journal of Experimental Hematology 2015;23(5):1459-1463
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of storage time on arginase level, and the possible source of arginase in suspended red blood cells (RBC).
METHODSThe arginase and myeloperoxidase (MPO) levels in suspended RBC and control plasma were detected by ELISA. The free hemoglobin level in suspended RBC and control plasma were detected by colorimetric method. The relationship between arginase level, MPO level and free hemoglobin level in suspended RBC was analyzed by the related methods.
RESULTSThe arginase and free hemoglobin levels in suspended RBC were higher than those in control plasma. Otherwise, MPO level was not significantly different between suspended RBC and control plasma. All of them did not increase along with prolonging of storage time. There was not a significant correlation between arginase level and free hemoglobin level in suspended RBC of different storage time (r = 0.03), but arginase level positively correlated with MPO level in the suspended RBC of different storage time (r = 0.76).
CONCLUSIONThe arginase level in suspended RBC storaged for different time increases significantly, but not along with prolonging of storage time. The main possible source of arginase in the suspended RBC is the residual white blood cell, especially neutrophils.
