Frequency of HLA-Cw and Its Corresponding Killer Cell Immunoglobulin-like Receptors (KIR) among Blood Donors in Chinese Nanjing Han Ethnic Group.

Hong Lin; Ling MA; Min Xue; Yan-chun Liu

Return

Frequency of HLA-Cw and Its Corresponding Killer Cell Immunoglobulin-like Receptors (KIR) among Blood Donors in Chinese Nanjing Han Ethnic Group.

Author: Hong LIN ¹ ; Ling MA ¹ ; Min XUE ¹ ; Yan-Chun LIU ²
Author Information

1. Blood Center of Jiangsu Province, Nanjing 210042, Jiangsu Province, China.
2. Blood Center of Jiangsu Province, Nanjing 210042, Jiangsu Province, China. E-mail: lyc60@126.com.
Publication Type:Journal Article
MeSH: Alleles; Asian Continental Ancestry Group; genetics; Blood Donors; China; Ethnic Groups; genetics; Genotype; HLA-C Antigens; genetics; Humans; Polymerase Chain Reaction; Polymorphism, Genetic; Receptors, KIR; genetics
From: Journal of Experimental Hematology 2015;23(5):1469-1473
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the frequency of HLA-Cw and its KIR2D genotypes in Han blood donor population in Chinese Nanjing area and to analyze the match and distinguish modes of them so as to provide the basis for further studying their roles in incidence and development of disease.
METHODSThe PCR-SSP was used to genotyping of HLA-Cw and KIR2D for 241 Han blood donors in Jiangsu Provincial blood center; according to distingush modes of HLA-Cw and KIR2D genes, the distingushed results of HLA-Cw and corresponding activating or inhibitory KIR2D receptors of individuals were analyzed.
RESULTSThe frequency of HLA-C1 expression in donor population of Nanjing area was 76.35% which was much higher than that of HLA-C2 expression (23.65%); the expression C1/C2 alleles was accorded with Handy-weinberg balance. The expression frequencies of 5 KIR2Ds (L1, L2, L3, S1 and S2) matched to HLA-Cw were 97.93%, 29.05%, 98.34%, 29.05% and 21.16%, respectively. The match of HLA-C1/C2 to 2DL1⁺/2DL2⁻/2DL3⁺/2DS1⁻/2DS2⁻ was predominated (75/241). Couclusion: The polymorphism data of HLA-Cw and 5 KIR2Ds from blood donors in Chinese Nanjing area has been provided in this study. The match analysis found that the expression of inhibitory HLA-Cw-KIR is higher than that of activated HLA-Cw-KIR, suggesting that the HLA-Cw/KIR2D combination is characterized by preponderance of inhibitory signal pathway.