Influence of artificial pneumoperitoneal media on colon carcinoma cell proliferation in vitro.
- Author:
Yong-Gang TIAN
1
;
Yin-Hua TANG
;
Jun XU
;
Wen-Jie DAI
;
Su LIU
;
Jin-Peng ZHAO
;
Yong MA
;
Hong-Chi JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; metabolism; pathology; Flow Cytometry; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Pneumoperitoneum, Artificial; methods
- From: Chinese Journal of Gastrointestinal Surgery 2007;10(6):561-564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the influence of different pneumoperitoneal media on colon carcinoma LS-174T cell proliferation in vitro.
METHODSThe artificial pneumoperitoneum was established. The proliferation of LS-174T cells was detected by MTT assay and soft agar clone formation assay. Expression of HIF-1alpha and VEGF was examined by immunohistochemistry. Apoptosis of LS-174T cells was analyzed by AO/EB double fluorescein stain and flow cytometry.
RESULTSThe growth speed and proliferating capacity of LS-174T cells in CO(2) pneumoperitoneum group[A:0.37 +/- 0.02,formation (32.8 +/- 3.6)%] were significantly higher than those in control group [A:0.33 +/- 0.01,formation (28.4 +/- 2.3)%] and He group [A:0.30 +/- 0.01,formation (23.5 +/- 2.7)%], meanwhile the He group was the lowest (P<0.01). Positive expression of HIF-1alpha and VEGF in CO(2) and He artificial pneumoperitoneum up-regulated significantly as compared to control group(P<0.01), meanwhile the above expression was higher in CO(2) group (P<0.01). The G(0 )/G(1) ratio in CO(2) group was the lowest as compared to control group and He group (P<0.01), and G(0 )/G(1) ratio in He group was higher than that of control group(P<0.01). Aapoptosis rate in He group was the highest as compared with the other two groups(P<0.01).
CONCLUSIONCO(2) pneumoperitoneum has stronger effect on the proliferation of colon carcinoma cell LS-174T as compared to He pneumoperitoneum in vitro.
