Effect of mesenteric lymphatic duct ligation on the system inflammation during the intestinal ischemia-reperfusion.
- Author:
Gui-zhen HE
1
;
Liang-guang DONG
;
Xiao-yu CUI
;
Xue-feng CHEN
;
Hong SHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Inflammation; Intestinal Diseases; metabolism; microbiology; pathology; Intestines; blood supply; pathology; Ligation; Lymph Nodes; pathology; Lymphatic System; surgery; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury; metabolism; microbiology; pathology
- From: Chinese Journal of Gastrointestinal Surgery 2008;11(5):469-471
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo estimate the effect of the lymph duct ligation on systemic inflammatory factors and endotoxins during intestinal ischemia-reperfusion (I/R).
METHODSMale SD rats underwent occlusion of superior mesenteric artery for 60 min followed by reperfusion for 120 min plus lymph duct ligation or not. Forty rats were randomly divided into 4 groups: group A (blank); group B (sham); group C (intestinal I/R); group D (intestinal I/R plus lymph duct ligation). Mesenteric lymph nodes were harvested for standard bacteriologic cultures. The endotoxin, D-lactate, diamine oxidase (DAO), and cytokines in serum were detected.
RESULTSThe rates of bacterial translocation to mesenteric lymph nodes were 40% in group C and 20% in group D. No positive lymph node cultures were encountered in any of group A and B. The serum cytokines (except for sICAM-1) , D-lactate, DAO and endotoxin levels were lower in group D than those in group C (P<0.05), but both were higher than those in group A and B (P<0.05).
CONCLUSIONDuring intestinal I/R injury, blockage the lymph flow from gut into bloodstream decreases the levels of cytokines, and significantly attenuates the increase in intestinal permeability.
