Salidroside protects PC12 cells from HO-induced apoptosis via suppressing NOX2-ROS-MAPKs signaling pathway.
- Author:
Zhi-Lin QI
1
,
2
;
Yin-Hua LIU
;
Shi-Mei QI
;
Lie-Feng LING
;
Zun-Yong FENG
;
Qiang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspase 3; metabolism; Glucosides; pharmacology; Hydrogen Peroxide; MAP Kinase Signaling System; drug effects; Membrane Glycoproteins; metabolism; NADPH Oxidase 2; NADPH Oxidases; metabolism; Neuroprotective Agents; pharmacology; PC12 Cells; Phenols; pharmacology; Phosphorylation; Rats; Reactive Oxygen Species; metabolism
- From: Journal of Southern Medical University 2016;37(2):178-183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular mechanism by which salidroside protects PC12 cells from HO-induced apoptosis.
METHODSPC12 cells cultured in DMEM supplemented with 10% horse serum and 5% fetal bovine serum were pretreated with different doses of salidroside for 2 h and then stimulated with HOfor different lengths of time. The expression levels of PARP and caspase 3 and the phosphorylation of p38, ERK and JNK were determined with Western blotting. The cell nuclear morphology was observed after DAPI staining. The production of ROS was detected using a ROS detection kit, and the levels of gp91and p47in the membrane and cytoplasm were detected by membrane-cytoplasm separation experiment; the binding between gp91and p47was assayed by coimmunoprecipitation experiment.
RESULTSSalidroside dose-dependently suppressed cell apoptosis, lowered phosphorylation levels of p38, ERK and JNK, inhibited the production of ROS, reduced the binding between gp91and p47, and inhibited the activity of NOX2 in PC12 cells exposed to HO.
CONCLUSIONSalidroside protects PC12 cells from HO-induced apoptosis at least partly by suppressing NOX2-ROS-MAPKs signaling pathway.
