Effects of retinol on expressions of epidermal growth factor, stem cell factor, colony-stimulating factor 1 and leukemia inhibitory factor in human umbilical cord-derived mesenchymal stem cells.
- Author:
Hua-Li ZHUO
1
;
Li-Peng BAI
;
Dan LIU
;
Shu-Min YU
;
Dan-Ting LI
;
Qian LIU
;
Pin SONG
;
Sui-Zhong CAO
;
Liu-Hong SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cells, Cultured; EGF Family of Proteins; metabolism; Humans; Immunophenotyping; Leukemia Inhibitory Factor; metabolism; Macrophage Colony-Stimulating Factor; metabolism; Mesenchymal Stromal Cells; drug effects; metabolism; Stem Cell Factor; metabolism; Umbilical Cord; cytology; Vitamin A; pharmacology
- From: Journal of Southern Medical University 2016;37(2):221-225
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate effects of retinol on the expressions of epidermal growth factor (EGF), stem cell factor (SCF), colony-stimulating factor 1 (CSF1) and leukemia inhibitory factor (LIF) in cultured human umbilical-derived mesenchymal stem cells (UCMSCs).
METHODSHuman UCMSCs were isolated from human umbilical cord and identified for immunophenotypes. The cells were then cultured in DMEM/F12 media supplemented with 12% fetal bovine serum (FBS), 12% FBS+1 µmol/L retinol, 15% knockout serum replacement (KSR) and 15% KSR+ 1 µmol/L retinol. The expressions of the cytokines EGF, SCF, CSF1 and LIF in the cells were detected using RT-PCR and ELISA.
RESULTSThe isolated cells exhibited characteristic immunophenotypes of human UCMSCs and expressed EGF, CSF1 and SCF at both mRNA and protein levels but not LIF protein. Retinol (1 µmol/L) significantly promoted the expressions of SCF and CSF1 at both mRNA and protein levels but did not result in changes of EGF and LIF expressions in human UCMSCs.
CONCLUSIONRetinol at the concentration of 1 µmol/L can promote expression of SCF and CSF1 in human UCMSCs in vitro.
