RNA interference of HERC4 inhibits proliferation, apoptosis and migration of cervical cancer Hela cells.
- Author:
Min WEI
1
;
Yan-Ling ZHANG
;
Lan CHEN
;
Cui-Xia CAI
;
Han-Duo WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cyclin D1; metabolism; Down-Regulation; Female; HeLa Cells; Humans; Proto-Oncogene Proteins c-bcl-2; metabolism; RNA Interference; RNA, Small Interfering; genetics; Transfection; Ubiquitin-Protein Ligases; genetics; metabolism; Uterine Cervical Neoplasms; pathology
- From: Journal of Southern Medical University 2016;37(2):232-237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of silencing HERC4 on the proliferation, apoptosis, and migration of cervical cancer cell line Hela and the possible molecular mechanisms.
METHODSThree HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells, and HERC4 expression in the cells was examined with Western blotting. CCK-8 assay, annexin V-FITC/PI assay, and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation, apoptosis and migration ability of Hela cells. The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.
RESULTSTransfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P<0.01). HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells, increased the apoptosis rate (P<0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P<0.01).
CONCLUSIONSilencing of HERC4 efficiently inhibits the proliferation, migration, and invasion of Hela cells in vitro, and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.
