Effect of HBP-A on meniscal injury and pathological hypertrophy and calcification of the meniscus.
- Author:
Guo-Qing DU
1
,
2
;
Dao-Fang DING
;
Yuan-Yuan FENG
;
Ling-Hui LI
;
Teng-Fei LEI
;
Bo CHEN
;
Zhen DENG
;
Hong-Sheng ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcinosis; drug therapy; Cattle; Glucans; pharmacology; Hypertrophy; Menisci, Tibial; drug effects; Osteoarthritis, Knee; drug therapy; Real-Time Polymerase Chain Reaction; Tibial Meniscus Injuries; drug therapy
- From: Journal of Southern Medical University 2016;37(4):431-437
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of HBP-A on meniscal injuries and the expressions of genes associated with pathological hypertrophy and calcification of the meniscusinduced by abnormal loading.
METHODSBovine meniscus explants were subjected to 25% strain at 0.3 Hz for 3 h and treated with 0.6 mg/mL of HBP-A. The cell viability in the meniscus explants after 72 hin culture was determined using live/dead staining and the expression levels of genes associated with pathological hypertrophy and calcification of the meniscus (ANKH, ENPP1, ALP, MMP13, and IL-1) were measured using real-time PCR and Western blotting. The conditioned medium was collected for testing sulfated glycosaminoglycan (GAG) release.
RESULTSThe number of dead cells, loss of proteoglycan content, and the expressions of ANKH, ENPP1, ALP and MMP13, and IL-1 at both the mRNA and protein levels were all significantly lower in the meniscus explants treated with 0.6 mg/mL HBP-A than in the explants with only 25% abnormal pressure stimulation (n=3, P<0.05).
CONCLUSIONHBP-A can effectively alleviate meniscal injuries induced by abnormal loading and suppress the expressions of genes related with pathological hypertrophy and calcification of the meniscus, and can serve as a potential drug for treatment of knee osteoarthritis.
