Expressions of inflammatory and fibrogenic factors in perinephric and subcutaneous adipose tissues of patients with adrenocorticotropic hormone-independent Cushing's syndrome.
- Author:
Chun-Yan WU
1
;
Hui-Jian ZHANG
;
Cun-Xia FAN
;
Peng WU
;
Qiang WEI
;
Ying-Ying CAI
;
Shao-Zhou ZOU
;
Ling WANG
;
Yao-Ming XUE
;
Mei-Ping GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adrenalectomy; Adrenocorticotropic Hormone; CCAAT-Enhancer-Binding Protein-beta; metabolism; Cushing Syndrome; metabolism; surgery; Early Growth Response Protein 1; metabolism; Humans; Matrix Metalloproteinase 2; metabolism; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; metabolism; Real-Time Polymerase Chain Reaction; Subcutaneous Fat; metabolism; Tissue Inhibitor of Metalloproteinase-1; metabolism; Tumor Necrosis Factor-alpha; metabolism; Uncoupling Protein 1; metabolism
- From: Journal of Southern Medical University 2016;37(4):563-566
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of inflammation- and fibrosis-related genes in perinephric and subcutaneous adipose tissues in patients with adrenocorticotropic hormone (ACTH)-independent Cushing's syndrome.
METHODSThe perinephric and subcutaneous adipose tissues adipose tissues were obtained from 8 patients with ACTH-independent Cushing's syndrome undergoing laparoscopic retroperitoneal adrenalectomy. Real-time PCR was used to detect the mRNA expression levels of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), matrix metallopeptidase 2 (MMP-2), TIMP metallopeptidase inhibitor 1 (TIMP-1), early growth response 1 (EGR1), CCAAT/enhancer binding protein β(CEBPβ), uncoupling protein 1(UCP-1), PPARγ coactivator 1 alpha (PGC1α) and cell death-inducing DFFA-like effector a (CIDEA).
RESULTSThe mRNA level of CIDEA was significantly higher in the perinephric adipose tissue (peri-N) than in the subcutaneous adipose tissue (subQ) (P<0.05). The expressions of CEBPβ, UCP-1, and PGC1α mRNA in the peri-N were similar with those in the subQ. The expressions of IL-6, TIMP1 and EGR1 mRNA in the subQ were significantly higher than those in the peri-N (P<0.05). No significant difference in TNF-α and MMP-2 mRNA levels was found between peri-N and subQ.
CONCLUSIONThe expression levels of the inflammation- and fibrosis-related genes are higher in the subQ than in the peri-N of patients with ACTH-independent Cushing's syndrome, suggesting that chronic exposure to endogenous hypercortisolism may cause adipose tissue dysfunction.
