Effect of gushen pelyuan recipe on Bcl-2 and Bax mRNA expressions in cochlea tissue of rats with Shen-yang deficiency syndrome.
- Author:
Li LI
1
;
Jun-Feng WANG
;
Da-Jing XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cochlea; metabolism; Diagnosis, Differential; Drugs, Chinese Herbal; therapeutic use; Hearing Loss, Sensorineural; drug therapy; metabolism; Kidney Diseases; drug therapy; metabolism; Male; Medicine, Chinese Traditional; Phytotherapy; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Yang Deficiency; drug therapy; metabolism; bcl-2-Associated X Protein; genetics; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(6):533-536
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Gushen Peiyuan Recipe (GPR) on the expression of Bcl-2 and Bax mRNA in Shen-yang-deficiency (SYD) rats suffering from cochlea apoptosis, thus providing a theoretical basis to the treatment and prevention of sensorineural hearing loss and fill Chinese medine's theory of kidney-ear-correlation with new substance.
METHODSRats were induced into experimental SYD animal models by injecting cetacort into their buttocks. Rats in the blank and model groups were given 10 mL/kg normal saline by gastrogavage, and 31 g/kg, 15.5 g/kg and 7.5 g/kg GPR were given to the rats in the high, medium and low dose groups by gastrogavage respectively. RT-PCR was adopted to detect the mRNA expressions of Bcl-2 and Bax.
RESULTSLevels of Bcl-2 mRNA expression and Bcl-2/Bax enhanced, and mRNA expression of Bax attenuated in the model rats after GPR treatment, the Bcl-2/Bax ratio increased, showing insignificant difference when compared with the blank control group (P > 0.05).
CONCLUSIONGPR plays a significant role in regulating the mRNA expressions of Bcl-2 and Bax, therefore improving the hearing of SYD rats and protecting the structure and function of cochlea.
