The effects of complement C3f segment on expression and secretion of collagen I, III and transforming growth factor-beta1 in human embryonic lung fibroblast.
- Author:
Wei LIU
1
;
Qing-Bo MA
;
Juan-Juan CHEN
;
Hai-Xia KONG
;
Mao-Ti WEI
;
Shi-Xin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Collagen Type I; metabolism; Collagen Type III; metabolism; Complement C3b; pharmacology; Fibroblasts; drug effects; metabolism; Humans; Lung; cytology; drug effects; embryology; Transforming Growth Factor beta1; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(1):61-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of complement fragment C3f on expression and secretion of collagen I, III and transforming growth factor( TGF)-beta1 in human embryonic lung fibroblast (MRC-5) cells.
METHODSMRC-5 cells were cultured with C3f (the synthetic 17 peptides fragments of complement C3). The extracellular and intracellular expression levels of type I, III collagens and TGF-beta1 in MRC-5 cultures were detected by ELISA and immunohistochemistry, respectively.
RESULTSThe expression levels of type I, III collagen and TGF-beta1 in the supernatant of MRC-5 cultures decreased significantly with the concentrations of C3f as compared with controls (P < 0.05). Also the expression level of TGF-beta1 in MRC-5 cytoplasm reduced significantly as compared with controls (P < 0.05).
CONCLUSIONThe results of present in vitro study showed that the complement fragment C3f could reduce the formation of TGF-beta1 and type I, III collagens in MRC-5 cells, and inhibit the lung tissue fibrosis.
