Application of BIOMED-2 primers in immunoglobulin gene rearrangement analysis of ocular adnexal lymphoma: a pilot study.
- Author:
Yu-chuan WANG
1
;
Li-ming WANG
;
Peng HAO
;
Ming YING
;
Rui-fang HAN
;
Jin-yong LIN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; Adult; Aged; DNA Primers; Eye Neoplasms; diagnosis; genetics; pathology; Female; Gene Rearrangement, B-Lymphocyte, Heavy Chain; Gene Rearrangement, B-Lymphocyte, Light Chain; Humans; Lymphoma, B-Cell; diagnosis; genetics; pathology; Lymphoma, Follicular; diagnosis; genetics; pathology; Male; Middle Aged; Paraffin Embedding; Pilot Projects; Young Adult
- From: Chinese Journal of Pathology 2010;39(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR.
METHODSDNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-actin. IgH(B) and IgK(B) primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software.
RESULTS76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements.Positive detection rates by BIOMED-2 IgH(B) and IgK(B) primers were 79.2% (38/48) and 68.8% (33/48), respectively, with a combined positive detection rate of 91.7% (44/48).
CONCLUSIONSIgH(B) and IgK(B) primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.
