Effects of 17β-estradiol on proliferation and migration of MCF-7 cell by regulating expression of claudin-6.
- Author:
Ya-fang LIU
1
;
Qiong WU
;
Xiao-ming XU
;
Yue REN
;
Li-na YU
;
Cheng-shi QUAN
;
Yu-lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Movement; drug effects; Cell Proliferation; drug effects; Claudins; Dose-Response Relationship, Drug; Estradiol; administration & dosage; pharmacology; Female; Humans; Membrane Proteins; metabolism
- From: Chinese Journal of Pathology 2010;39(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of estrogen in the regulation of the expression of claudin-6 and biological behavior in MCF-7 cells.
METHODSRT-PCR and immunocytochemistry were conducted to analyze the expression and localization of claudin-6 in MCF-7 cells treated with 17β-estradiol. CCK-8 kit assay and Scratch Test were conducted to analyze the capability of proliferation and migration of 17β-estradiol treated MCF-7 cells.
RESULTSRT-PCR analysis and immunocytochemistry showed that 17β-estradiol induced a concentration-and time-dependent effect on claudin-6. At 5 nmol/L and at 24 h, 17β-estradiol treatment led to an increased level of claudin-6, which was located in the membrane of MCF-7 cells. CCK-8 analysis showed a significant decrease in the capability of proliferation of MCF-7 cells compared with the control group (P < 0.05). Cells Scratch Test showed decreased migration capability of MCF-7 cells compared with the control group (P > 0.05).
CONCLUSIONS17β-E2 might regulate the expression of claudin-6 and inhibit the proliferation and migration of MCF-7 cells. The inhibitory effects of 17β-E2 on growth and migration of MCF-7 cells may be mediated by claudin-6 expression regulation.
