Effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts.
- Author:
Bin-bin LI
1
;
Shi-feng YU
;
Shu-zhen PANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Differentiation; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Cells, Cultured; Genistein; pharmacology; Osteoblasts; cytology; drug effects; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Stomatology 2005;40(3):237-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts in vitro.
METHODSThe primary osteoblasts (OBs) were obtained from the rat calvaria and the cell line of osteosarcoma-UMR-106 served as control. The cells in the experiment group were grown in 10% (volume fraction) fetal calf serum (FCS) + alphaMEM + various concentrations of genistein. The control groups were grown in 10% FCS + alphaMEM. The growth of OBs was assessed by flow cytometry and MTT method. The differentiation of OBs was examined by the alkaline phosphatase (ALP) activity.
RESULTSFlow cytometry analysis and MTT showed that genistein could prompt primary OB from stage G(0) (G(1)) to stage S, G(2) or M. By contrast, genistein had no effect on the cell cycle of UMR-106, but could induce its apoptosis. Additionally, the results of ALP activity showed that genistein stimulated the differentiation of primary OB.
CONCLUSIONSGenistein can stimulate the proliferation and differentiation of the primary osteoblasts in some degree, and induce the apoptosis of osteosarcoma cells.
