Identification and isolation of human dental pulp stem cells.
- Author:
Xue-chao YANG
1
;
Ming-wen FAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cell Differentiation; Cell Separation; methods; Cells, Cultured; Dental Pulp; cytology; Humans; Stem Cells; cytology; Young Adult
- From: Chinese Journal of Stomatology 2005;40(3):244-247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and cultivate human dental pulp stem cells (DPSCs).
METHODSPulp tissue was removed from healthy young human teeth extracted for orthodontic purposes. The pulp was digested by Type I collagenase and dispase. Then single-cell suspensions were obtained by filter and cultivated. The clones were identified by expression of STRO-1. Under the conditions of inducement, clones were identified by activity of alkaline phosphatase (ALP), formation of mineralized nodule and expression of dentin sialoprotein (DSP), and by Oil Red-O dyeing and expressing of PPARr2.
RESULTSThe clones had positive expression of STRO-1. When stimulated to differentiation, these cells took on dramatically high activity of ALP, had the ability of mineralization and expressed DSP. These cells also had ability to trans-differentiate into adipocytes.
CONCLUSIONThere are stem cells in human dental pulp tissues, which can be isolated and cultivated.
