Preliminary study on anti-periodontitis immunization with DNA vaccine.
- Author:
Feng-qiu ZHANG
1
;
Lian-jia YANG
;
Zhi-fen WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Vaccines; immunology; therapeutic use; Immunoglobulin A, Secretory; analysis; Periodontitis; immunology; microbiology; prevention & control; Porphyromonas gingivalis; genetics; immunology; Rats; Rats, Sprague-Dawley; Vaccines, DNA; immunology; therapeutic use
- From: Chinese Journal of Stomatology 2005;40(4):310-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the protection against periodontal bone loss in the Sprague-Dawley (SD) rats periodontitis model, with the recombined plasmid pcDNA3.1+/kgpcd as DNA gene vaccine.
METHODSPcDNA3.1+/kgpcd was delivered into rats by submandibular gland-targeted injection. The anti-KGPcd sIgA in saliva was measured by indirect ELISA method. Immunohistochemistry staining was used to assess the protection in the animal model.
RESULTSThe level of specific anti-KGPcd sIgA in saliva of the experimental group was significantly higher than that of control group. HE staining showed that immunization with recombined plasmid pcDNA3.1+/kgpcd could protect or minimize tissue destruction caused by subsequent P. gingivalis challenge in the rat model.
CONCLUSIONSThe results indicate that pcDNA3.1+/kgpcd was a good candidate for anti-periodontitis gene vaccine and could provide protection against Porphyromonas gingivalis-caused periodontitis in rat lesion model.
