Identification and characterization of synovial mesenchymal stem cells in temporomandibular joint.

Author: Jian LI ¹ ; Xing LONG ; Jin KE ; Qing-gong MENG ; Wei FANG
Author Information

1. Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Publication Type:Journal Article
MeSH: Cell Differentiation; Cell Separation; methods; Cells, Cultured; Flow Cytometry; Humans; Mesenchymal Stromal Cells; cytology; Synovial Membrane; cytology; Temporomandibular Joint
From: Chinese Journal of Stomatology 2005;40(5):362-364
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo identify and characterize mesenchymal stem cells from synovial membrane of temporomandibular joint in vitro.
METHODSSynovial mesenchymal stem cells (SMSCs) were obtained by limited dilution method and expanded in 25 ml flasks. Methyl thiazolyl tetrazolium (MTT) method was used to determine the cell growth cycles. The expressions of vimentin and keratin were respectively detected with immunocytochemistry, while the expressions of CD8, CD34, CD44, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by flow cytometry.
RESULTSPure mesenchymal stem cells were of spindle shape and uniform in size, which were intensively positive in vimentin, but negative in keratin. The expression of CD44, VCAM-1 and ICAM-1 were also verified by flow cytometry.
CONCLUSIONSMesenchymal stem cells could be purified from adult synovial membrane of temporomandibular joint.