Effects of 17 beta-estradiol on the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
- Author:
De-sheng WEN
1
;
Jun-zheng WU
;
Shan-min FU
;
Bin LIU
;
Xiao-xia LI
;
Ji-jun DONG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Mucoepidermoid; metabolism; pathology; Cell Adhesion; drug effects; Cell Line, Tumor; Estradiol; pharmacology; Humans; Neoplasm Invasiveness; Neoplasm Metastasis; Salivary Gland Neoplasms; metabolism; pathology
- From: Chinese Journal of Stomatology 2005;40(5):390-393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of 17 beta-estradiol on the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
METHODSThe effects of 17 beta-estradiol on adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells were investigated with cell attachment assay on fibronectin (FN), wound assay, chemotaxis assay, and gelatin-incorporated SDS-PAGE electrophoresis. The expression of estrogen receptor in Mc3 cells was determined by immunohistochemistry assay.
RESULTSAttachment rates of Mc3 cells treated with E2 at 10(-9), 10(-8), 10(-7), 10(-6) mol/L were 38.3%, 50.4%, 69.2% and 91.1% respectively, and the rate in control was 25.0%. When exposed to 17 beta-estradiol at 10(-9), 10(-8), 10(-7) and 10(-6) mol/L for 48 h, motility of Mc3 cells on FN increased by 16.9%, 40.9%, 36.4% and 38.8% respectively. When at 10(-6) mol/l, 17 beta-estradiol increased chemotaxis potential of Mc3 cells to FN by 60.3%. The activity of 68 000 matrix metalloproteinase (MMP-2) of Mc3 cells was enhanced at different levels by 10(-9), 10(-8), 10(-7), 10(-6) mol/L of 17 beta-estradiol, and estrogen receptor was also detected in nucleus of Mc3 cells by immunohistochemistry assay.
CONCLUSIONS17 beta-estradiol at physiological concentration may enhance the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
