Study on the identification characteristics of rRNA genes on Yersinia pestis
10.3760/cma.j.issn.0254-6450.2011.04.010
- VernacularTitle:鼠疫耶尔森菌rRNA基因识别特征研究
- Author:
Hui-Juan ZHANG
1
;
Jian-Chun WEI
;
En-Min ZHANG
;
Xiao-Na SHEN
;
Ying LIANG
;
Xiao-Yu ZHU
;
Dong-Lei XU
;
Li-Na SUN
;
Jian-Hua ZHANG
;
Dong-Zheng YU
;
Rong HAI
Author Information
1. 中国疾病预防控制中心传染病预防控制所
- Keywords:
Yersinia pestis;
rRNA genes;
Identification characteristics
- From:
Chinese Journal of Epidemiology
2011;32(4):366-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the identification characteristics of rRNA genes on Yersinia (Y.)pestis.Methods By means of comparative genomics,we compared the rRNA genome sequences of nine completely sequenced strains of Y. pestis isolated from China and other countries by Clustal W software.we also compared the 2000 bp sequence adjacent to the rRNA genes,rRNA genes and 16S-23S rRNA spacer region respectively to determine the identification features of rRNA genes for Y. pestis.Results There were 6 rRNA gene clusters in the strains of D182038,D106004,Z176003 and CO92 respectively(6 copies strain).There were 7 rRNA gene clusters in the strains of 91001,KIM,Nepa1516,Antiqua and Pestoides F(7 copies strain).According to the 2000 bp sequence,13 types of rRNA gene clusters could classify the strains between the 6 copies and 7 copies.There were 4 types of tRNA gene among the 16S-23S rRNA spacer region that could classify the strains among the 6 copies and 7 copies strains respectively.The number of point mutation among the 23S rRNA gene was statistically different in some copies under ANOVA analysis(F=0.548,P=0.815>0.05 among the strains and F=5.228,P<0.01 among the copies).Conclusion The 2000 bp sequence adjacent to the rRNA genes,tRNA gene and 23S rRNA gene sequence could serve as the identification sign of rRNA genes for classifing the strains of Y. pestis.
