Therapeutic Effect of Amniotic Membrane Extract on Keratitis Following Corneal Alkali Burn.
- Author:
Sang Woo HA
1
;
Jae Soon KIM
;
Tae Bum CHEONG
;
Jae Chan KIM
Author Information
1. Department of Ophthalmology, College of Medicine, Chung-Ang University.
- Publication Type:Original Article
- Keywords:
Amniotic membrane extract;
Corneal alkali burn;
Wound healing
- MeSH:
Alkalies*;
Amnion*;
Burns*;
Cornea;
Corneal Opacity;
Follow-Up Studies;
Keratitis*;
Methylcellulose;
Ophthalmic Solutions;
Rabbits;
Transplants;
Wound Healing;
Wounds and Injuries
- From:Journal of the Korean Ophthalmological Society
2001;42(11):1555-1561
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Amniotic membrane (AM) contains various proteinase inhibitors and when used as a graft, it could enhance healing process by blocking insult of inflammatory cells and inhibiting proteolytic damage. Thus we evaluated whether applying of amniotic membrane extract as eyedrops could get the same effect as amniotic membrane patching. METHODS: Alkali wounds were inflicted on the central corneas of rabbits by applying a round filter paper, 6.0 mm in diameter, soaked in 1N NaOH for 30 seconds. A total of 16 rabbits were divided into four groups: (1) applied with amniotic membrane extract; (2) applied with amniotic membrane extract and Healon(R); (3) applied with methylcellulose; and (4) control. Each material was applied for 1 week. During follow-ups, epithelial defects, corneal thickness and its opacity were measured. RESULTS: The epithelial healing was faster and the corneal thickness was thinner in amniotic membrane extract applied groups than in non-applied. Corneal opacity was much less in AM extract applied groups. CONCLUSIONS: AM extract as eyedrops promotes wound healing and it could be an effective method for treating various keratitis due to its convenience and good effect.
